pi staining solution (Beyotime)
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Beyotime
pi staining solution
Pi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hoechst+33342+staining+solution/Hoechst+33342+Staining+Solution/pm41786017-104-14-17
Average 99 stars, based on 461 article reviews
Pi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hoechst+33342+staining+solution/Hoechst+33342+Staining+Solution/pm41786017-104-14-17
Average 99 stars, based on 461 article reviews
pi staining solution - by Bioz Stars,
2026-10
99/100 stars
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Incubation:Article Title: ATP6V1B2 alleviates hepatic steatosis by promoting lysosomal acidification in hepatocytes. Article Snippet: Immunofluorescence Cells were fixed with 4% paraformaldehyde for 15min, washed with PBS, and permeabilized with 0.1% Triton X-100 (P0096, Beyotime, China) for 10min. after washing with PBS, the cells were closed with 5% BSA (BS114100g, Biosharp, China) for 1 h. The cells were then incubated with primary antibody at 4 °C overnight. .. Subsequently, the samples were washed with PBS, and the fluorescent secondary antibody was incubated in a dark environment at room temperature for 1 h. Finally, the cells were stained with Article Title: Myeloperoxidase (MPO) mediates LPS-induced mitophagy in murine macrophages through AMPK/ULK1 pathway. Article Snippet: Neutrophils play a key role in innate immune system, taking up 50%70% of the whole circulating leukocytes, which are considered as the body's first line defense against pathogens.. Once reach the injured site, active neutrophils could release myeloperoxidase (MPO) through degranulation or neutrophil extracellular traps (NETs), making MPO a hallmark for neutrophil activation [1].. As a heme-containing peroxidase, MPO could kill pathogens through catalyzing H2O2 and halide ions (Cl , Br , I and SCN ) to produce oxidative hypohalides, among which hypochlorous acid (HOCl) is the main product in vivo [2,3]. Article Title: ATP6V1B2 alleviates hepatic steatosis by promoting lysosomal acidification in hepatocytes Article Snippet: Cells were fixed with 4% paraformaldehyde for 15 min, washed with PBS, and permeabilized with 0.1% Triton X-100 (P0096, Beyotime, China) for 10 min. after washing with PBS, the cells were closed with 5% BSA (BS114-100g, Biosharp, China) for 1 h. The cells were then incubated with primary antibody at 4 °C overnight. .. Subsequently, the samples were washed with PBS, and the fluorescent secondary antibody was incubated in a dark environment at room temperature for 1 h. Finally, the cells were stained with Staining:Article Title: ATP6V1B2 alleviates hepatic steatosis by promoting lysosomal acidification in hepatocytes. Article Snippet: Immunofluorescence Cells were fixed with 4% paraformaldehyde for 15min, washed with PBS, and permeabilized with 0.1% Triton X-100 (P0096, Beyotime, China) for 10min. after washing with PBS, the cells were closed with 5% BSA (BS114100g, Biosharp, China) for 1 h. The cells were then incubated with primary antibody at 4 °C overnight. .. Subsequently, the samples were washed with PBS, and the fluorescent secondary antibody was incubated in a dark environment at room temperature for 1 h. Finally, the cells were stained with Article Title: Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity decline Article Snippet: .. Following the instructions of the mitochondrial membrane potential and apoptosis detection kit with MitoTracker Red CMXRos and Annexin V-FITC, 188 μL of Annexin V-FITC binding solution, 5 μL of Annexin V-FITC, 2 μL of MitoTracker Red CMXRos staining solution, and 5 μL of Article Title: Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity decline. Article Snippet: .. Following the instructions of the mitochondrial membrane potential and apoptosis detection kit with MitoTracker Red CMXRos and Annexin V-FITC, 188 μL of Annexin V-FITC binding solution, 5 μL of Annexin V-FITC, 2 μL of MitoTracker Red CMXRos staining solution, and 5 μL of Article Title: Myeloperoxidase (MPO) mediates LPS-induced mitophagy in murine macrophages through AMPK/ULK1 pathway. Article Snippet: Neutrophils play a key role in innate immune system, taking up 50%70% of the whole circulating leukocytes, which are considered as the body's first line defense against pathogens.. Once reach the injured site, active neutrophils could release myeloperoxidase (MPO) through degranulation or neutrophil extracellular traps (NETs), making MPO a hallmark for neutrophil activation [1].. As a heme-containing peroxidase, MPO could kill pathogens through catalyzing H2O2 and halide ions (Cl , Br , I and SCN ) to produce oxidative hypohalides, among which hypochlorous acid (HOCl) is the main product in vivo [2,3]. Article Title: ATP6V1B2 alleviates hepatic steatosis by promoting lysosomal acidification in hepatocytes Article Snippet: Cells were fixed with 4% paraformaldehyde for 15 min, washed with PBS, and permeabilized with 0.1% Triton X-100 (P0096, Beyotime, China) for 10 min. after washing with PBS, the cells were closed with 5% BSA (BS114-100g, Biosharp, China) for 1 h. The cells were then incubated with primary antibody at 4 °C overnight. .. Subsequently, the samples were washed with PBS, and the fluorescent secondary antibody was incubated in a dark environment at room temperature for 1 h. Finally, the cells were stained with Article Title: ATP6V1B2 alleviates hepatic steatosis by promoting lysosomal acidification in hepatocytes Article Snippet: Using LysoTracker Red (C1046, Beyotime, China) probe diluted at 1:5000 in serum-free medium, cells were added and incubated for 40 min at 37 °C in the dark. .. After washing with PBS, the cells were fixed with glutaraldehyde (PH9003, Feijin Research, China) and then stained with Article Title: Atorvastatin Attenuates Vancomycin-Induced Nephrotoxicity via PPARα-Associated Regulation of SLC Transporters Article Snippet: Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (DMEM/F-12), fetal bovine serum (FBS), penicillin streptomycin antibiotic mixture, and PPARα inhibitor GW6471 purchased from Sigma Chemical, Co. (St. Louis, MO, USA). .. The Reactive Oxygen Species (ROS) Detection Kit, Article Title: ATP6V1B2 alleviates hepatic steatosis by promoting lysosomal acidification in hepatocytes. Article Snippet: Using LysoTracker Red (C1046, Beyotime, China) probe diluted at 1:5000 in serum-free medium, cells were added and incubated for 40min at 37 °C in the dark. .. After washing with PBS, the cells were fixed with glutaraldehyde (PH9003, Feijin Research, China) and then stained with Membrane:Article Title: Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity decline Article Snippet: .. Following the instructions of the mitochondrial membrane potential and apoptosis detection kit with MitoTracker Red CMXRos and Annexin V-FITC, 188 μL of Annexin V-FITC binding solution, 5 μL of Annexin V-FITC, 2 μL of MitoTracker Red CMXRos staining solution, and 5 μL of Article Title: Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity decline. Article Snippet: .. Following the instructions of the mitochondrial membrane potential and apoptosis detection kit with MitoTracker Red CMXRos and Annexin V-FITC, 188 μL of Annexin V-FITC binding solution, 5 μL of Annexin V-FITC, 2 μL of MitoTracker Red CMXRos staining solution, and 5 μL of Binding Assay:Article Title: Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity decline Article Snippet: .. Following the instructions of the mitochondrial membrane potential and apoptosis detection kit with MitoTracker Red CMXRos and Annexin V-FITC, 188 μL of Annexin V-FITC binding solution, 5 μL of Annexin V-FITC, 2 μL of MitoTracker Red CMXRos staining solution, and 5 μL of Article Title: Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity decline. Article Snippet: .. Following the instructions of the mitochondrial membrane potential and apoptosis detection kit with MitoTracker Red CMXRos and Annexin V-FITC, 188 μL of Annexin V-FITC binding solution, 5 μL of Annexin V-FITC, 2 μL of MitoTracker Red CMXRos staining solution, and 5 μL of Infection:Article Title: Myeloperoxidase (MPO) mediates LPS-induced mitophagy in murine macrophages through AMPK/ULK1 pathway. Article Snippet: Neutrophils play a key role in innate immune system, taking up 50%70% of the whole circulating leukocytes, which are considered as the body's first line defense against pathogens.. Once reach the injured site, active neutrophils could release myeloperoxidase (MPO) through degranulation or neutrophil extracellular traps (NETs), making MPO a hallmark for neutrophil activation [1].. As a heme-containing peroxidase, MPO could kill pathogens through catalyzing H2O2 and halide ions (Cl , Br , I and SCN ) to produce oxidative hypohalides, among which hypochlorous acid (HOCl) is the main product in vivo [2,3]. Fluorescence:Article Title: ATP6V1B2 alleviates hepatic steatosis by promoting lysosomal acidification in hepatocytes Article Snippet: Using LysoTracker Red (C1046, Beyotime, China) probe diluted at 1:5000 in serum-free medium, cells were added and incubated for 40 min at 37 °C in the dark. .. After washing with PBS, the cells were fixed with glutaraldehyde (PH9003, Feijin Research, China) and then stained with Article Title: ATP6V1B2 alleviates hepatic steatosis by promoting lysosomal acidification in hepatocytes. Article Snippet: Using LysoTracker Red (C1046, Beyotime, China) probe diluted at 1:5000 in serum-free medium, cells were added and incubated for 40min at 37 °C in the dark. .. After washing with PBS, the cells were fixed with glutaraldehyde (PH9003, Feijin Research, China) and then stained with Microscopy:Article Title: ATP6V1B2 alleviates hepatic steatosis by promoting lysosomal acidification in hepatocytes Article Snippet: Using LysoTracker Red (C1046, Beyotime, China) probe diluted at 1:5000 in serum-free medium, cells were added and incubated for 40 min at 37 °C in the dark. .. After washing with PBS, the cells were fixed with glutaraldehyde (PH9003, Feijin Research, China) and then stained with Article Title: ATP6V1B2 alleviates hepatic steatosis by promoting lysosomal acidification in hepatocytes. Article Snippet: Using LysoTracker Red (C1046, Beyotime, China) probe diluted at 1:5000 in serum-free medium, cells were added and incubated for 40min at 37 °C in the dark. .. After washing with PBS, the cells were fixed with glutaraldehyde (PH9003, Feijin Research, China) and then stained with TUNEL Assay:Article Title: Atorvastatin Attenuates Vancomycin-Induced Nephrotoxicity via PPARα-Associated Regulation of SLC Transporters Article Snippet: Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (DMEM/F-12), fetal bovine serum (FBS), penicillin streptomycin antibiotic mixture, and PPARα inhibitor GW6471 purchased from Sigma Chemical, Co. (St. Louis, MO, USA). .. The Reactive Oxygen Species (ROS) Detection Kit, |
